rabbit polyclonal anti ctmp  (Cell Signaling Technology Inc)

Journal: Journal of Cellular Physiology

Article Title: Carboxyl Terminal Modulator Protein Induces Cell Senescence and Is Upregulated With Aging by Zic2 in Rats

doi: 10.1002/jcp.70007

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti‐CTMP , Cell Signaling Technology , 4612s.

Techniques: Plasmid Preparation, Luciferase, Transfection, Staining, Protease Inhibitor

Journal: Molecular neurobiology

Article Title: Age-related up-regulation of carboxyl terminal modulator protein contributes to the decreased brain ischemic tolerance in older rats

doi: 10.1007/s12035-017-0826-6

Figure Lengend Snippet: (A) Representative images of immunolabeling of NeuN (green) and CTMP (red). Magnification x 200. Scale bar equals to 50 μm. (B) Representative images of immunolabeling of NeuN (green) and Akt phosphorylated at Ser473 (p-Ser473-Akt) (red). Magnification x 200. Scale bar equals to 50 μm. (C) Representative images and quantification of Western blot of CTMP. (D) Representative images and quantification of Western blot of Akt phosphorylated at Thr308. (E) Representative images and quantification of Western blot of Akt phosphorylated at Ser473. (F) Representative images and quantification of Western blot of GSK-3β phosphorylated at Ser9. Data are mean ± S.E.M. (n = 6–9). * P < 0.05 compared with 2-month old rats.

Article Snippet: The membranes were blocked with Protein-Free T20 Blocking Buffer (catalog number: 37573, Thermo Scientific) and incubated with the following primary antibodies overnight at 4°C: rabbit polyclonal antibody to Akt (1:1000, catalog number: 9272; Cell Signaling), mouse monoclonal antibody to p-S473-Akt (1:1000, catalog number: 12694; Cell Signaling), mouse monoclonal antibody to p-T308-Akt (1:1000, catalog number: 5106 Cell Signaling;), rabbit polyclonal antibody to CTMP (1:1000, catalog number: 4612; Cell Signaling), rabbit monoclonal antibody to p-S9-GSK-3β (1:1000, catalog number: 9323; Cell Signaling), mouse monoclonal antibody to GSK-3β (1:1000, catalog number:9832; Cell Signaling), and rabbit polyclonal antibody to β-actin (1:1000, catalog number: 4967; Cell Signaling).

Techniques: Immunolabeling, Western Blot

Journal: Molecular neurobiology

Article Title: Age-related up-regulation of carboxyl terminal modulator protein contributes to the decreased brain ischemic tolerance in older rats

doi: 10.1007/s12035-017-0826-6

Figure Lengend Snippet: (A) Representative images and quantification of Western blot of CTMP. (B) Representative images and quantification of Western blot of Akt phosphorylated at Thr308. (C) Representative images and quantification of Western blot of Akt phosphorylated at Ser473. (D) Representative images and quantification of Western blot of GSK-3β phosphorylated at Ser9. Data are mean ± S.E.M. (n = 6). * P < 0.05 compared with 2-month old rats. ^ P < 0.05 compared with 6-month old rats.

Article Snippet: The membranes were blocked with Protein-Free T20 Blocking Buffer (catalog number: 37573, Thermo Scientific) and incubated with the following primary antibodies overnight at 4°C: rabbit polyclonal antibody to Akt (1:1000, catalog number: 9272; Cell Signaling), mouse monoclonal antibody to p-S473-Akt (1:1000, catalog number: 12694; Cell Signaling), mouse monoclonal antibody to p-T308-Akt (1:1000, catalog number: 5106 Cell Signaling;), rabbit polyclonal antibody to CTMP (1:1000, catalog number: 4612; Cell Signaling), rabbit monoclonal antibody to p-S9-GSK-3β (1:1000, catalog number: 9323; Cell Signaling), mouse monoclonal antibody to GSK-3β (1:1000, catalog number:9832; Cell Signaling), and rabbit polyclonal antibody to β-actin (1:1000, catalog number: 4967; Cell Signaling).

Techniques: Western Blot

Journal: Molecular neurobiology

Article Title: Age-related up-regulation of carboxyl terminal modulator protein contributes to the decreased brain ischemic tolerance in older rats

doi: 10.1007/s12035-017-0826-6

Figure Lengend Snippet: Viruses carrying code for CTMP miRNA and non-targeting (NT) miRNA were injected into cerebral cortical tissues 14 days before the brain tissues were harvested or before the rats were subjected to a 30 min MCAO. (A) Representative images and quantification of Western blot of CTMP. (B) Representative images and quantification of Western blot of Akt phosphorylated at Ser473. Results for panels A and B are mean ± S.E.M. (n = 6–10). * P < 0.05 compared with control and NT-miRNA groups. (C) Representative brain sections stained with 2,3,5-triphenyltetrazolium chloride. (D) Quantification of brain infarct volume. (E) Performance of rats on rotarod. Results in panels D and E are mean ± S.E.M. (n = 6–8). (F) Neurological deficit scores presented in box plot (n = 8 to 21). ^ P < 0.05 compared with 2-month old rats with MCAO only. # P < 0.05 compared with 6-month old rats with MCAO only. Ve: vehicle, LY: LY294002.

Article Snippet: The membranes were blocked with Protein-Free T20 Blocking Buffer (catalog number: 37573, Thermo Scientific) and incubated with the following primary antibodies overnight at 4°C: rabbit polyclonal antibody to Akt (1:1000, catalog number: 9272; Cell Signaling), mouse monoclonal antibody to p-S473-Akt (1:1000, catalog number: 12694; Cell Signaling), mouse monoclonal antibody to p-T308-Akt (1:1000, catalog number: 5106 Cell Signaling;), rabbit polyclonal antibody to CTMP (1:1000, catalog number: 4612; Cell Signaling), rabbit monoclonal antibody to p-S9-GSK-3β (1:1000, catalog number: 9323; Cell Signaling), mouse monoclonal antibody to GSK-3β (1:1000, catalog number:9832; Cell Signaling), and rabbit polyclonal antibody to β-actin (1:1000, catalog number: 4967; Cell Signaling).

Techniques: Injection, Western Blot, Control, Staining

Journal: Molecular neurobiology

Article Title: Age-related up-regulation of carboxyl terminal modulator protein contributes to the decreased brain ischemic tolerance in older rats

doi: 10.1007/s12035-017-0826-6

Figure Lengend Snippet: Rats with or without receiving viruses carrying code for CTMP miRNA and non-targeting (NT) miRNA in the cerebral cortical tissues 14 days before the brain tissues were subjected to a 30 min left MCAO. Their left Fr1 was harvested 24 h later. (A) and (B) Representative images and quantification of Western blot of CTMP, phosphorylated Akt and phosphorylated GSK-3β. (C), (D), (E) and (F) Representative images and quantification of Western blot of CTMP, phosphorylated Akt and phosphorylated GSK-3β. Results for panels B, D, E and F are mean ± S.E.M. (n = 6–8). # P < 0.05 compared with 2-month old rats. * P < 0.05 compared with control. ^ P < 0.05 compared with control and NT-miRNA groups.

Article Snippet: The membranes were blocked with Protein-Free T20 Blocking Buffer (catalog number: 37573, Thermo Scientific) and incubated with the following primary antibodies overnight at 4°C: rabbit polyclonal antibody to Akt (1:1000, catalog number: 9272; Cell Signaling), mouse monoclonal antibody to p-S473-Akt (1:1000, catalog number: 12694; Cell Signaling), mouse monoclonal antibody to p-T308-Akt (1:1000, catalog number: 5106 Cell Signaling;), rabbit polyclonal antibody to CTMP (1:1000, catalog number: 4612; Cell Signaling), rabbit monoclonal antibody to p-S9-GSK-3β (1:1000, catalog number: 9323; Cell Signaling), mouse monoclonal antibody to GSK-3β (1:1000, catalog number:9832; Cell Signaling), and rabbit polyclonal antibody to β-actin (1:1000, catalog number: 4967; Cell Signaling).

Techniques: Western Blot, Control